Esophageal Adenocarcinoma Methods and Protocols
176 Zhao Y, Bao Q, Schwarz B, Zhao L, Mysliwietz J, Ellwart J, Renner A, Hirner H, Niess H, Camaj P, Angele M, Gros S, Izbicki ...
177 Alfred K. Lam (ed.), Esophageal Adenocarcinoma: Methods and Protocols, Methods in Molecular Biology, vol. 1756, https://doi. ...
178 origin of multiple tumors existing in the same person.” While the presence of CTCs has been reported for over 100 years, the ...
179 Epithelial–mesenchymal transition (EMT) is one of the bio- logical processes involved in the genesis of CTC from primary tum ...
180 metastatic phenotypes. This association is strengthened by the findings by Reeh et al., where they have demonstrated that pa ...
181 comparison of survival rates between two histological subgroups in these studies. These study results indicate that CTCs pos ...
182 blood sample is subjected to filtration through polycarbonate membranes presenting 8 μm pores, we can separate the CTCs. Fig ...
183 2 Materials Anti-EpCAM ferrofluid which contains a suspension of 0.022% magnetic particles conjugated to a mouse monoclonal ...
184 activated cell sorting (FACS) analysis. This two- step procedure will reassure the quality of molecular analysis in these CT ...
185 References Ashworth T (1869) A case of cancer in which cells similar to those in the tumours were seen in the blood after d ...
186 patients with non-small-cell lung cancer. J Clin Oncol 29:1556–1563 Sclafani F, Smyth E, Cunningham D, Chau I, Turner A, Wa ...
187 Alfred K. Lam (ed.), Esophageal Adenocarcinoma: Methods and Protocols, Methods in Molecular Biology, vol. 1756, https://doi. ...
188 all of which can be detected in the circulation [ 3 – 5 ]. The use of liquid biopsy thus provides a minimally invasive way t ...
189 management of patients with esophageal adenocarcinoma. The ability for cfDNA and mutations within to be detected consistentl ...
190 Refrigerated centrifuge capable of at least 14,000 × g. Refrigerated ultracentrifuge capable of at least 105,000 × g. 0.22 ...
191 Immediately upon collection, gently invert collection tube five times to mix blood. Be careful not to shake or treat the bl ...
192 Centrifuge solution at 4 °C at 1000 × g for 15 min. Move supernatant to a new tube with sufficient additional vol- ume for ...
193 Centrifuge isolated plasma at 4 °C at 1000 × g for 10 min fol- lowed by centrifugation at 4 °C at 10,000 × g for 30 min. Re ...
194 4 Notes Plasma samples from multiple collection tubes can be pooled for increased DNA yields. In order to prevent contamina ...
195 Alfred K. Lam (ed.), Esophageal Adenocarcinoma: Methods and Protocols, Methods in Molecular Biology, vol. 1756, https://doi. ...
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