Esophageal Adenocarcinoma Methods and Protocols
156 Measure subcutaneous tumor size (tumor length, L and width, W) with digital caliper twice a week for 2–4 weeks (see Note 27 ...
157 Perform mice survival study [ 12 , 29 ] with paclitaxel and car- boplatin treatments (Fig. 3 ) [ 12 ]. Inject 10 × 106 esop ...
158 0 0 50 Cumulative survival 100 20 (^4653) 57 ControlCarboplatin∗ Median survival (Days) Paclitaxel∗ 40 60 Time (Days) Fig. 3 ...
159 GlutaMax is an improved cell culture supplement of l- glutamine that is stable at room temperature and used in cell culture ...
160 G2/M phase arrest and mitotic cell death by tubulin polymer- ization and stabilization of microtubules [ 32 – 34 ]. Carbopla ...
161 Do frequent agitations of cell suspensions in the tube before drawing up into the syringe for intraperitoneal injection. Th ...
162 Snap freeze of tumor tissues in liquid nitrogen is necessary for protein and RNA extraction, whereas formaldehyde fixation ...
163 Barrett’s adenocarcinoma in a mouse xenograft model. Mol Cancer Ther 12(3):255–263. https://doi.org/10.1158/1535-7163. mct-1 ...
164 perceived-but they can be improved. Cancer Biol Ther 2(4 Suppl 1):S134–S139 Gros SJ, Kurschat N, Dohrmann T, Reichelt U, Da ...
165 Alfred K. Lam (ed.), Esophageal Adenocarcinoma: Methods and Protocols, Methods in Molecular Biology, vol. 1756, https://doi. ...
166 A combination of techniques such as cell sorting based on cell surface and intracellular protein marker, in vitro functional ...
167 CSCs are responsible for intrinsic and acquired chemotherapy resistant to 5-fluorouracil (5-FU) and cisplatin in esophageal ...
168 Fig. 1 Isolation and identification of CSCs from esophageal adenocarcinoma. SP-positive cells are separated by fluorescence- ...
169 media to the water (see Note 1). Stir until it completely dis- solves and do not heat the media (see Note 2). Weigh 2.0 g of ...
170 Cell culture plates: 6-well plates, 96-well ultralow attachment plates. Cell culture media: RPMI1640 (see Subheading 2.1). ...
171 Grow the esophageal adenocarcinoma cells up to 70–80% con- fluency and harvest the cells after trypsinization. Take the fla ...
172 Select the gates for sorting into external collection tubes (see Note 20). Run the experimental sample tubes at 4 °C (see N ...
173 4 Notes Powdered media are highly hygroscopic and should be pro- tected from atmospheric moistures. Preparing a concentrate ...
174 cheal tube. Be careful not to overanesthetize, as the mice will succumb to respiratory depression. Thawing procedures are s ...
175 tremorgin C. Cells treated with inhibitor are used to set gate and therefore can discriminate SP population from non-SP cell ...
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