Science - USA (2022-06-10)
featured with well-resolved side chains of a large number of bulky residues (Fig. 4A, figs. S5 and S10). Nup93-ACE1-O resides at ...
where the tail of Nup96-I contacts the crown of Nup107-O and stacks against the N-terminal a-solenoid of clamp 1 (fig. S14A, mid ...
Discussion A wealth of structural information at atomic resolution on the NPC have emerged from crystal structures of individual ...
(corresponding to segmentsa37 toa47 in the structure) of Nup160 were missing in a patient with steroid-resistant nephrotic syndr ...
geometrical restraint among subunits of the same CR and five rounds of guided multiref- erence three-dimensional classification. ...
pixel size of 2.774 Å. These particles were directly subjected to two additional rounds of two-dimensional classifications in RE ...
functional orthologs of Nup93 and the Alpha- Fold model. The EM maps also allow identifi- cation of protein-protein interfaces m ...
P. Emsley, K. Cowtan, Coot: Model-building tools for molecular graphics.Acta Crystallogr. D Biol. Crystallogr. 60 , 2126– 2132 ...
RESEARCH ARTICLE SUMMARY ◥ NUCLEAR PORE COMPLEX Architecture of the cytoplasmic face of the nuclear pore Christopher J. Bley†, S ...
RESEARCH ARTICLE ◥ NUCLEAR PORE COMPLEX Architecture of the cytoplasmic face of the nuclear pore Christopher J. Bley^1 †, Si Nie ...
Bleyet al., Science 376 , eabm9129 (2022) 10 June 2022 2of18 Fig. 1. Reconstitution of a 16-proteinC. thermophilumcomplex compos ...
therapeutic strategies for these conditions remain ill defined. Here we present insight into the atomic and higher-order archite ...
Bleyet al., Science 376 , eabm9129 (2022) 10 June 2022 4of18 Fig. 2. Conserved modular architecture and RNA-binding properties o ...
we carried out a comprehensive electrophoretic mobility shift assay (EMSA) screen to systemat- ically assess binding against a c ...
sides of the NPC in the form of distinct Ran- binding domains (RanBDs) and Zn2+-finger (ZnF) modules. On the cytoplasmic face, N ...
previous reports, the RanBDs of NUP358 and NUP50 only bound Ran(GTP), whereas the ZnFs in NUP358 and NUP153 bound Ran in both nu ...
OE regions, whereas all other domains were dispensable (Fig. 5B and fig. S62). When NUP358NTDor NUP358OEwere tested in iso- lati ...
incubation periods andachieved only a partial NUP358 knockdown, potentially allowing sec- ondary phenotypes to emerge from prolo ...
to 100% of cells displayed a strong nuclear 5-EU–labeled RNA signal that decreased over time with a concomitant increase in the ...
NUP160::NG-AIDDLD1 cells after depletion of NUP160, the knockout of which causes RNA retention inS. cerevisiae,demonstrating the ...
«
7
8
9
10
11
12
13
14
15
16
»
Free download pdf