Science - USA (2022-06-10)
cargo (Fig. 6, B and C, and fig. S83A). However, the NUP214NTD•DDX19 complex forms tighter interactions when DDX19 is in its ADP ...
Placement of the CFNC hub into the tube- like density puts CCS1, CCS2, and likely the unresolved CCS3 within reach of the ~40-Å ...
architectural outcome: TheC. thermophilum NPC presents two distinct assembly sensor motifs for the CFNC hub in the Nup37 and Nup ...
face cryo-ET density, accounting for ~23 MDa of structured mass. The more flexibly attached regions of the CF nups that are not ...
outstanding challenge for structural determi- nation. Nevertheless, our analysis has already identified that competition for bin ...
A. E. Hodelet al., The three-dimensional structure of the autoproteolytic, nuclear pore-targeting domain of the human nucleopor ...
635 – 652 (2000). doi:10.1083/jcb.148.4.635; pmid: 10684247 J. M. Cronshaw, A. N. Krutchinsky, W. Zhang, B. T. Chait, M. J. Mat ...
RESEARCH ARTICLE SUMMARY ◥ NUCLEAR PORE COMPLEX Structure of cytoplasmic ring of nuclear pore complex by integrative cryo-EM and ...
RESEARCH ARTICLE ◥ NUCLEAR PORE COMPLEX Structure of cytoplasmic ring of nuclear pore complex by integrative cryo-EM and AlphaFo ...
nuclease to remove contaminating chromatin (fig. S1A). Cryo-EM data collection was con- ducted at different stage tilts and in c ...
Fontanaet al., Science 376 , eabm9326 (2022) 10 June 2022 3of11 Fig. 2. Fitting of Y-complex Nups with AlphaFold.(A) Cryo-EM den ...
the Nup358 N-terminal region as having a large⍺-helical domain (~800 residues), a linker, and an isolated single⍺-helix (Fig. 4, ...
Fontanaet al., Science 376 , eabm9326 (2022) 10 June 2022 5of11 Fig. 3. Interactions mediated by Nup205 and the Nup214-Nup88-Nup ...
of the CR assembly that was unexpected is the observed asymmetry in the composition and mode of binding among Nups: the con- for ...
Fontanaet al., Science 376 , eabm9326 (2022) 10 June 2022 7of11 Fig. 4. Nup358 interacts with the Y-complexes as clamps.(A) Doma ...
Cryo-EM data processing Data processing leveraged computer support from the SBgrid Consortium ( 59 ). Movies were corrected by g ...
subtraction using a CR protomer mask, and these subtracted particles were recentered and boxsizere-windowedto300by300pixels,all ...
shape. Inner and outer Nup205 assume a differ- ent position due to the presence of the Nup214- Nup88-Nup62 complex in the inner ...
Mol. Cell. Biol. 24 , 2373–2384 (2004). doi:10.1128/ MCB.24.6.2373-2384.2004; pmid: 14993277 W. Kühlbrandt, Biochemistry. The r ...
RESEARCH ARTICLE SUMMARY ◥ NUCLEAR PORE COMPLEX AI-based structure prediction empowers integrative structural analysis of human ...
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