Science - USA (2022-06-10)
RESEARCH ARTICLE ◥ NUCLEAR PORE COMPLEX AI-based structure prediction empowers integrative structural analysis of human nuclear ...
IR subunits, and inter-ring connectors. We observe an increase in the distance between the adjacent spokes within the IR, in agr ...
(fig. S14). In the case of the NUP214 complex, for which no structures are available, the ColabFold model is highly consistent w ...
membrane-binding N-terminal domains of NUP155 and amphipathic helices of NUP35, which is also consistent with our proximity labe ...
during dilation, rather the relative distances within the spokes remain constant while the spacing of the spokes increases (Fig. ...
specific physiological conditions ( 23 ). Our model includes multiple previously unassigned do- mains and proteins, resolves lon ...
reveals that the structured regions are gener- ally modeled with good confidence, while linkers and peripheral loops are less we ...
Subtomograms (7711) containing individual NPCs were extracted, corresponding to 61,688 asymmetric units. The pixel size at the s ...
pixel size of 0.730 Å per pixel. Movies were acquired at a dose rate of 4.95 electrons per pixel per second, and a total dose of ...
To calculate the percentage of the molecular weight of the full NPC and the NPC scaffold covered by the new and the old models, ...
S ¼diagðÞDP= 3 ;DP= 3 ; 2 DP= 3 whereDP ≡ P⊥P∥:The resulting tension on the double- membrane system iss¼ðÞP⊥P∥Lz¼DPLz withLz ...
A.-R. Choet al., Tissue-specific expression and subcellular localization of ALADIN, the absence of which causes human triple A ...
conceptualized the study, supervised the project, and wrote the manuscript. CRediT roles: Conceptualization: S.M., A.O.-K., G.H. ...
RESEARCH ARTICLE SUMMARY ◥ NUCLEAR PORE COMPLEX Architecture of the linker-scaffold in the nuclear pore Stefan Petrovic, Dipanja ...
RESEARCH ARTICLE ◥ NUCLEAR PORE COMPLEX Architecture of the linker-scaffold in the nuclear pore Stefan Petrovic, Dipanjan Samant ...
channel, linkers bridge the layers of the in- ner ring to coalesce scaffold nups into eight relatively rigid spokes that are fle ...
Petrovicet al., Science 376 , eabm9798 (2022) 10 June 2022 3of18 Fig. 2. Structural and biochemical analyses of the Nup192-Nic96 ...
and fig. S2, A and D). The Nic96R2sequence register was unambiguously assigned by iden- tifying seleno-L-methionine (SeMet)–labe ...
Petrovicet al., Science 376 , eabm9798 (2022) 10 June 2022 5of18 Fig. 3. Structural and biochemical analyses of the Nup192-Nup14 ...
Petrovicet al., Science 376 , eabm9798 (2022) 10 June 2022 6of18 Fig. 4. Structural and biochemical analyses of the Nup188-Nup14 ...
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