Food Biochemistry and Food Processing

194 Table 8.4. Comparison of the Main Expression Systems Mammalian Transgenic Transgenic Bacteria Yeast Fungi Insect Cells Cells ...

8 Enzyme Engineering and Technology 195 lipolytica(Madzak et al. 2004). As in bacteria, ex- pression in yeast relies on episomal ...

196 Part II: Water, Enzymology, Biotechnology, and Protein Cross-linking of a few eukaryotes that have circular nuclear plas- mi ...

8 Enzyme Engineering and Technology 197 fibrinogen (Prunkard et al. 1996) have been pro- duced in transgenic animals, and there ...

198 Part II: Water, Enzymology, Biotechnology, and Protein Cross-linking Figure 8.13.(A) Schematic diagram of a chromatogram sho ...

8 Enzyme Engineering and Technology 199 pH and ionic strength conditions suitable for target protein binding; (2) proteinsample ...

200 Part II: Water, Enzymology, Biotechnology, and Protein Cross-linking to maximize the conditions required for the forma- tion ...

8 Enzyme Engineering and Technology 201 products, cofactors, inhibitors, or allosteric effectors are all potential candidates as ...

202 Part II: Water, Enzymology, Biotechnology, and Protein Cross-linking 2003; Labrou et al. 2004). These biomimetic dyes exhibi ...

8 Enzyme Engineering and Technology 203 more efficient catalysts (Chen et al. 1999, Born- scheuer and Pohl 2001, Zaks 2001, Pank ...

204 Part II: Water, Enzymology, Biotechnology, and Protein Cross-linking system has to be established. Introduction of the cDNA ...

8 Enzyme Engineering and Technology 205 cleotide-directed mutagenesis) in order for the mismatched oligonucleotide to anneal. Th ...

206 Part II: Water, Enzymology, Biotechnology, and Protein Cross-linking Figure 8.18.PCR oligonucleotide-directed mutagenesis. T ...

8 Enzyme Engineering and Technology 207 Although site-directed mutagenesis is widely used, it is not always feasible due to the ...

208 Part II: Water, Enzymology, Biotechnology, and Protein Cross-linking Currently, directed evolution has gained consider- able ...

8 Enzyme Engineering and Technology 209 enzymes to insoluble supports, due to their wide- spread surface exposure and high react ...

210 Part II: Water, Enzymology, Biotechnology, and Protein Cross-linking Various types of beaded supports have been used success ...

8 Enzyme Engineering and Technology 211 gelatin are usually used to provide additional pro- tein molecules as spacers (Podgornik ...

212 Part II: Water, Enzymology, Biotechnology, and Protein Cross-linking the interstitial spaces of lattice. Many materials, for ...

8 Enzyme Engineering and Technology 213 in routine laboratory practice, and others that are used in clinical laboratory assays. ...