Front Matter

13.3.1 Immobilization techniques The immobilization of single enzymes for one-step reactions or whole cells for more complex rea ...

weak interaction forces the application of enzymes immobilized in this way often suffers from a considerable protein leakage, li ...

factor regeneration. The cofactor molecules, e.g., NADH are prevented from pene- trating the membrane by enlarging their molecul ...

cells ofStreptomyces lydicusfor the continuous production of PLD in an air-lift reactor. The synthesis of polymers with a wide v ...

covalent binding; a surface area of about 100 m^2 g–1in order to guarantee a sufficient protein capacity with a pore size of app ...

Diffusion limitation An important quality criterion of a carrier applied to enzyme immobilization is its protein capacity. Thus, ...

10 kJ mol–1(Buchholz and Ru ̈th, 1976; Lasch, 1979). More detailed reports on this theme have been published by Bisswanger (1994 ...

of phospholipases as such. Here, the influence of experimental parameters (described in Sections 13.3.1–13.3.3) on the propertie ...

affect the activity of either form of the enzyme in case of low diheptanoyl-PE con- centrations. The data obtained with diheptan ...

an indication for the adsorption of PLA 2 in its native state, and for the absence of diffusion limitation. The activities of na ...

area of about 400 m^2 g–1bearing ethylenediamine groups on their surface (Deloxan DAP III; Table 2) suitable for operating in aq ...

the adsorption of PLA 2 from aqueous solutions to a polysiloxane carrier with a hy- drophobic surface (Deloxan HAP) was examined ...

A special type of application of immobilization techniques is that of enzyme pur- ification by affinity chromatography. Bernal a ...

378 C, whereas the degree of PE- and PC- hydrolysis was about 50 % after 60 h under the same conditions. Since the early 1980s m ...

13.4.2 Immobilization of phospholipase D Possible applications of immobilized PLD are the synthesis of new phospholipids via tra ...

for the determination of PLD-activity contained 0.625 mM PC, 15 mM SDS, 0.3 M sodium acetate buffer (pH 5.1), and 40 mM CaCl 2. ...

new phospholipids was demonstrated by preparing corresponding compounds where the choline residue was exchanged by glucose (68 % ...

days’ storage under the same conditions), and a considerable broadening of the pH- optimum compared to the soluble PLD resulting ...

Because of the increasing interest in using PLDs as catalysts for the synthesis of new phospholipids (D’Arrigo and Servi, 1997; ...

glycerophosphorylcholine + H 2 OPLD! glycerol-3-phosphate + choline (1) glycerol-3-phosphateG-3-PO!dihydroxyacetonephosphate ...