Front Matter

or N425M exchange (A424 and N425 of the human 5-LOX align with I418 and M419 of the rabbit and human 15-LOX) transformed the wil ...

Optimizing arachidonate lipoxygenating species for biotechnological applications Current knowledge on the structural determinant ...

AA with acceptable yields (Reddanna et al., 1990). This enzyme does not require essential co-factors, and is stable over months ...

In addition, it has been shown for soybean LOX-1 that its positional specificity against LA is affected by a pH-dependent mechan ...

Figure 3B) by interacting with a basic residue at the bottom of the substrate-binding cleft (see Figure 3B, residue B). 15.4 For ...

15.5 References Began, G., Sudharshan, E., Appu Rao, A.G. (1999), Change in the positional specificity of lipoxygenase 1 due to ...

Fleming, J., Thiele, B.J., Chester, J., O’Prey, J., Janetzki, S., Aitken, A., Anton, I.A., Rapoport, S.M., Harrison, P.R. (1989) ...

Ku ̈hn, H., Belkner, J., Wiesner, R., Brash, A.R. (1990a), Oxygenation of biological membranes by the pure reticulocyte lipoxyge ...

Piazza, G.J. (1996), Immobilization of soybean lipoxygenase and promotion of fatty acid ester oxidation, in Piazza, G.J. (Ed.),L ...

16 Properties and Applications of Lipoxygenases Gilles Iacazio and Dominique Martini-Iacazio Abbreviations 13(S)-H(P)ODE (9Z,11E ...

16.1 Introduction Lipoxygenases (LOXs) are nonheme iron dioxygenases (Galliard and Chan, 1980; Vliegenthart and Veldink, 1982; V ...

PUFA-HPODs are potentially cytotoxic compounds as they can induce radical chain reactions that are deleterious to living organis ...

dedicated specifically to the enzymatic synthesis of PUFA-HPODs and, as stated by the authors, this work represented a major imp ...

tion of the various isomers was determined (Figure 3), 13(S)-HPODE being the major product of the reaction (96 %, 95 % ee). Four ...

A major feature of these experiments was that only low oxygen pressures were needed to conduct high-yield lipoxygenation. This p ...

Other methods such as microemulsion systems (Piazza, 1992) have been devel- oped to overcome the low solubility of PUFAs in wate ...

16.3 Substrate and product specificities of lipoxygenases 16.3.1 Soybean lipoxygenase-1 (SBLOX-1) Substrate specificity of SBLOX ...

The two substrates (x-3 andx-6) with a pentadienyl system positioned at thex-6 position were oxygenated by SBLOX-1 at the highes ...

Product specificity of SBLOX-1 with natural PUFAs Beside LA and AA being used widely as substrates of LOXs, other PUFAs such asa ...

Some years later, the Vliegenthart group re-examined the double dioxygenation of AA (Van Os et al., 1981). Along with the alread ...