Front Matter

Lipoxygenases Enzymesin LipidModification.Editedby UweT. Bornscheuer Copyright 2000 Wiley-VCHVerlagGmbH& Co. KGaA,Weinheim. ...

15 Application of Lipoxygenases and Related Enzymes for the Preparation of Oxygenated Lipids Ivo Feussner and Hartmut Ku ̈hn 15. ...

fatty acid isomers enzymatically since the diversity of LOX positional specificity is not sufficient. For instance, currently it ...

the resulting mobility of the substrate during catalysis might be designed specifically to promote release of free radical inter ...

chanistic data available, and it is rather difficult to prove or disprove either of these models experimentally. A key intermedi ...

15.3 The structural bases of the positional specificity of LOXs and alteration of the positional specificity by modifying the su ...

314 15 Application of Lipoxygenases and Related Enzymes Figure 2. Radical mechanism of the LOX reaction. ...

Earlier attempts to alter the direction of radical rearrangement during LOX reac- tion did not lead to active LOX mutants. Howev ...

be favored (Figure 3B, right-hand side). In addition, the stereochemistry of the LOX reaction can be explained perfectly with th ...

To investigate the role of other sequence determinants for the positional specificity of plant LOXs, we point-mutated the Borngr ...

318 15 Application of Lipoxygenases and Related Enzymes Figure 4. Straight-phase HPLC analysis of hydroxy polyenoic fatty acids ...

15.3 The structural bases of the positional specificity of LOXs 319 Table 2.Products formed from the reaction of wild-type lipid ...

320 15 Application of Lipoxygenases and Related Enzymes Figure 6. Reversed-phase HPLC analysis of oxidized TL derivatives formed ...

15.3.2 Specificity of mammalian LOXs In contrast to plant cells, most mammalian tissues contain large amounts of AA and in vario ...

of 12/15-LOXs with the methyl end (Ku ̈hn et al., 1986; Lehmann, 1994). According to this model (Figure 7) AA is oxygenated at C ...

* Introduction of the bulky and hydrophilic glycerol moiety at the carboxy-terminus reversed the effects of methylation. This ma ...

(14R,15S)-DiH(P)ETE by the soybean LOXs does not exclude an AA-like substrate orientation. It may be possible that only the inve ...

LOX. The exchanged region contained the amino acids I418 and M419, which had been previously characterized as primary determinan ...

mutagenesis of I593 (I593A) was carried out and the mutant enzyme converted AA to 12- and 15-HETE in a ratio of about 1 : 1 (Bor ...

are aligned at the active site, and whether the rules established for polyenoic fatty acids are also applicable for complex subs ...