Antibiotic Resistance Protocols (Methods in Molecular Biology)

Antibiotic Resistance Protocols Stephen H. Gillespie Editor Third Edition Methods in Molecular Biology 1736 ...

For further volumes: http://www.springer.com/series/ Me t h o d s i n Mo l e c u l a r Bi o l o g y Series Editor John M. Walker ...

Antibiotic Resistance Protocols Third Edition Edited by Stephen H. Gillespie School of Medicine, University of St. Andrews, Nort ...

ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7636-2 ISBN 978-1-4939-7638-6 (eBook) ht ...

v Since the first two editions in this series were published, much has changed in the world of antibiotic resistance research. I ...

vii Preface.............................................................. v Contributors........................................ ...

viii 11 Transcriptional Profiling Mycobacterium tuberculosis from Patient Sputa....... 117 Leticia Muraro Wildner, Katherine A. ...

ix Niels AgersNAp • Philips BioCell A/S, Allerød, Denmark MANuel AlcAlde-rico • Departamento de Biotecnología Microbiana, Centro ...

x sAM lipworth • Department of Tropical Medicine and Global Health, University of Oxford, Oxford, UK José luis MArtíNez • Depart ...

1 Stephen H. Gillespie (ed.), Antibiotic Resistance Protocols, Methods in Molecular Biology, vol. 1736, https://doi.org/10.1007/ ...

2 ations [ 4 ]. Among such changes, one of relevance for bacterial behavior consists on alterations in the quorum sensing (QS) r ...

3 family [ 27 ]. One of the best characterized AHLs signaling system is based on N-(3-oxo-dodecanoyl)-l-homoserine lactone (3-ox ...

4 2 Materials Pseudomonas aeruginosa PAO1 wild type. P. aeruginosa ΔpqsA. Escherichia coli pSB1705 (LasR-based bioreporter) [ 2 ...

5 50 ml, 100 ml and 250 ml glass flasks. HPLC glass tubes with cover. Centrifuge microtubes 1.5 ml. Centrifuge tubes 50 ml. Spe ...

6 cellular pellet will be used for the extraction of cell-associated autoinducers. Add 10 ml of the cell-free supernatant to a ...

7 ml (PqsR-based biosensor) [ 33 ] in 50 ml flasks with a single colony of each reporter strain from freshly grown agar plates w ...

8 TLC until the solvent reaches 2–4 cm from the top of the plate and let dry (see Note 17). Once the TLC plate obtained after S ...

9 Inoculate 10 ml of LB in a 50 ml flask with a single colony of each strain to be tested from freshly grown agar plates (see N ...

10 Next day, 5 μl from each sample (AHLs or AQs extract) is charged into each well of a microtiter plate. Include in the same p ...

11 To obtain a representative result, you have to repeat the extrac- tion and TLC-bioassay at least with three different biolog ...