Antibiotic Resistance Protocols (Methods in Molecular Biology)
77 demonstrated the suitability of the system to early detect the resis- tance profiles of bacteria reference strains and multid ...
78 2- aminoethanesulfonic acid (TES) (CAMHBT, Sensititre®, Thermo Scientific) [ 17 , 18 ]. ●● Transparent flat bottom microtiter ...
79 Inoculate the bacterial strain of interest in 3–5 mL of appropri- ate growth medium according to its metabolic and environ- ...
80 which would otherwise affect the quality of the acquired images and, hence, the quantitative analysis (see Note 1). Introduc ...
81 sible to set two different phase of analysis with different time intervals between sequential images. Such intervals can be a ...
82 ●● The Normalized version of each algorithm (Norm) is com- puted in the same way as the respective algorithm but the value of ...
83 be used as long as the sample fully covers the bottom of the well during the entire period of analysis. It is always recomme ...
84 Kjeldsen T, Sommer M, Olsen JE (2015) Extended spectrum β-lactamase-producing Escherichia coli forms filaments as an initial ...
85 Stephen H. Gillespie (ed.), Antibiotic Resistance Protocols, Methods in Molecular Biology, vol. 1736, https://doi.org/10.1007 ...
86 but often complex and may result in metabolic alteration in the cells studied. Separation of particles based on their buoyant ...
87 tiating between genera of bacteria [ 24 ]. It is rapid and effective and remains an important part of everyday microbiology p ...
88 When the preparation is visualized at 527 nm nonpolar lipids fluoresce green. Polar lipids such as those found in the cell wa ...
89 Add 600 μL of D 2 O to give final volume of 800 μL (D 2 O:dH 2 O; 3:1) (see Notes 4 and 5 ). Seal the D 2 O/dH 2 O solution ...
90 The supernatant is removed and discarded and 200 μL of 100% ethanol is added. Vortex to mix. The sample is centrifuged for 3 ...
91 range. We use Texas red and Bodipy FL cubes as these have a narrow spectral range, ±40 nm of the stated wavelength. This is ...
92 loaded into flow cytometry vessel and processed as normal. Microscopic analysis of Nile red stained preparations is recom- me ...
93 Greenspan P, Mayer EP, Fowler SD (1985) Nile red: a selective fluorescent stain for intra- cellular lipid droplets. J Cell B ...
94 Direct in situ viability assessment of bacteria in probiotic dairy products using viability stain- ing in conjunction with co ...
95 Stephen H. Gillespie (ed.), Antibiotic Resistance Protocols, Methods in Molecular Biology, vol. 1736, https://doi.org/10.1007 ...
96 [ 2 , 3 ] and the evolution of rifampicin resistance in M. tuberculosis, caused by mutations in rpoB, that is frequently asso ...
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