Antibiotic Resistance Protocols (Methods in Molecular Biology)
55 Larger volumes of cells can be stained by scaling up the quan- tity of dye used. For example, 1 mL of cells can be stained b ...
56 Acknowledgments Funding was received from Department of Health Grant in Aid and the National Institute of Health Research. Th ...
57 or the Department of Health. The research leading to this method also received funding from the Innovative Medicines Initiati ...
59 Stephen H. Gillespie (ed.), Antibiotic Resistance Protocols, Methods in Molecular Biology, vol. 1736, https://doi.org/10.1007 ...
60 continuous culture the bacteria are in equilibrium with their envi- ronment and growing at a constant generation time. While ...
61 Ty-Fast cable ties—141 mm (RS components Ltd., Corby, UK). Tubing connectors, Y shaped for 4–5 mm tubing ID (VWR Internation ...
62 Clips, tubing—30 mm (VWR International, Lutterworth, UK). Clips, tubing—40 mm (VWR International, Lutterworth, UK). Keck ram ...
63 using a 0.2 μm filter. Store the medium between 2 °C and 8 °C, in the dark and use within 2 months of the production date. Th ...
64 Phosphate buffered saline (PBS) pH 7.4 (Severn Biotech Ltd., Kidderminster, UK). CMM Mod2 medium (see Subheading 2.1 for the ...
65 Place the vessel on top of the stirrer and on the heat pad. Connect the probes to the correct channel on the Eycoferm contro ...
66 Return all probes to the chemostat and ensure that all con- nections through the head plate are tightened. Pressure-test the ...
67 Switch the culture to a fed-batch mode by starting the medium pump at a flow rate of 5 mL/h. Set medium pump by calibrat- in ...
68 Remove the PBS and repeat the wash and finally resuspend in PBS From the washed cell sample perform a tenfold dilution serie ...
69 Move the tubing through the pumps approximately every 2 weeks in order to maintain elasticity of the tubing and to reduce th ...
70 International, Lutterworth, UK, 229-0592. Clips, tub- ing—40 mm: VWR International, Lutterworth, UK, 229- 0593. Keck ramp cla ...
71 lent to 50% dissolved air saturation (DAT). The Eycoferm con- troller displays the DAT and not the DOT. It is not advisable ...
72 ing nutrient across the system. The relationship between the yield of cells using substrate, Yx/s (grams of biomass per gram ...
73 References Aldridge BB, Keren I, Fortune SM (2014) The spectrum of drug susceptibility in myco- bacteria. Microbiol Spectr 2 ...
75 Stephen H. Gillespie (ed.), Antibiotic Resistance Protocols, Methods in Molecular Biology, vol. 1736, https://doi.org/10.1007 ...
76 and quantitative results (e.g., determination of minimum inhibi- tory concentration) [ 1 , 13 ], although they may not accura ...
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