Antibiotic Resistance Protocols (Methods in Molecular Biology)
12 Be careful with the drying steps with N 2 to avoid splashing the walls and losing sample. If this happens, try to resuspend ...
13 The overnight cultures of biosensor strains have always to be diluted 1:100 into agar medium overlay for TLC bioassay. Befor ...
14 References Andersson DI, Levin BR (1999) The biological cost of antibiotic resistance. Curr Opin Microbiol 2:489–493. https: ...
15 Eng 10:145–167. https://doi.org/10.1146/ annurev.bioeng.10.061807.160536 Martínez JL (2014) Interkingdom signaling and its c ...
17 Stephen H. Gillespie (ed.), Antibiotic Resistance Protocols, Methods in Molecular Biology, vol. 1736, https://doi.org/10.1007 ...
18 their ease of transformation into bacterial cells, but are unfavorable for in vivo experiments due to the propensity for loss ...
19 Lysogeny broth (LB): 10 g/L tryptone, 5 g/L NaCl, 5 g/L yeast extract. Supplement with 15 g/L agar to make plates. Mix toget ...
20 Premixed BHI media made according to manufacturer’s instructions. Supplement with 15 g/L agar to make plates. Mix together w ...
21 Obtain vector plasmid DNA (see Note 3) using a commercial mini-prep kit. Digest approximately 1–2 μg of PCR and plasmid DNA ...
22 Leave at room temperature for 30 min, then repeat centrifugation. Resuspend in approximately 400 μL sterile, room temperatur ...
23 Incubate for 15 min at 37 °C with rapid aeration (e.g., 250 rpm). Use this time to prechill a centrifuge to 4 °C and place 2 ...
24 Defrost an aliquot of each strain on ice. Resuspend each aliquot in ice-cold PBS such that its final con- centration is 1 × ...
25 the host organism except for the introduction of a resistance cassette, consider carefully the orientation of the plasmid- bo ...
26 In S. aureus, standard antibiotic concentrations are 5 μg/mL tetracycline (Tet marker), 50 μg/mL kanamycin (Kan marker), or ...
27 References Law V, Knox C, Djoumbou Y et al (2013) DrugBank 4.0: shedding new light on drug metabolism. Nucleic Acids Res 42( ...
29 Stephen H. Gillespie (ed.), Antibiotic Resistance Protocols, Methods in Molecular Biology, vol. 1736, https://doi.org/10.1007 ...
30 evasion of the host immune system. In addition, many antibiotics are known to increase mutations rates in bacteria, which can ...
31 10 −^4 –10−^5 per nucleotide, DNA repair pathways reduce replicative error rates to 10−^9 –10−^11 per nucleotide [ 5 , 6 ]. M ...
32 2 Materials E. coli MG1655 expressing the xfp-mutL gene and deleted for the native chromosomal nonfluorescent mutL gene. The ...
33 be detected automatically using a simple thresholding method. We made two different chromosomal yfp-mutL constructs. In one c ...
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