Antibiotic Resistance Protocols (Methods in Molecular Biology)
97 for culture and drug dilutions throughout the assay. Make up according to the manufacturer’s instructions. Mueller-Hinton II ...
98 From a frozen stock, streak out wild-type E. coli MG1655 for single colonies on a Muller-Hinton agar plate. Incubate over- n ...
99 Transfer 900 μL of each overnight culture to 2 mL screw-cap tubes containing 225 μL of sterile 50% glycerol in Muller- Hinto ...
100 Plate format 8 × 12 (rows A–H, and columns 1–12). The for- matting of strains versus antibiotic concentrations can be plann ...
101 Transfer 50 μL of each bacteria suspension to 10 mL of MHII broth to make a set of final bacterial suspensions: ≅ 5 × 105 C ...
102 (>10^9 cells/mL for E. coli). In this case, either longer incuba- tions or smaller antibiotic concentration increases bet ...
103 Acknowledgments This work is supported by grants from the Swedish Research Council (VR-NT and VR-Med (not VE as written)-Med ...
105 Stephen H. Gillespie (ed.), Antibiotic Resistance Protocols, Methods in Molecular Biology, vol. 1736, https://doi.org/10.100 ...
106 The indigenous gastrointestinal microflora acts as a barrier against incoming pathogens and overgrowth of opportunistic micr ...
107 The indigenous intestinal flora acts as a barrier against incom- ing pathogens and overgrowth of opportunistic microorganism ...
108 Gel electrophoresis UV camera (Bio-Rad). A colonizing E. coli: Here, A clinical blood isolate of E. coli, lineage B2-O25b-S ...
109 (a) Suspend the CP in 0.9% saline to 10^8 CFU (colony form- ing units)/mL using a colorimeter (see Note 10). (b) Administer ...
110 After 18–20 h, calculate the CFU number for the resistant strain in question, the anaerobic Gram-negative flora and the aer ...
111 (e) Take a picture of the gel by your local UV gel electropho- resis camera. (f) The fingerprinting will differ in number of ...
112 resistant to one of the antibiotics used in the chromogenic agar and susceptible to the antibiotic used in blood agar and an ...
113 fold (10−^1 ), the third yet again diluted tenfold (10−^2 ), and so on (10−^3 to 10−^6 ). Spot-calculation: Average of the d ...
114 a reservoir for antibiotic-resistance determi- nants. J Med Microbiol 59:1331–1339 Kristiansson C, Grape M, Gotuzzo E et al ...
115 Zak O, Sande MA, O’Reilly T (1999) Handbook of animal models of infection. Academic, San Diego Erlendsdottir H, Knudsen JD, ...
117 Stephen H. Gillespie (ed.), Antibiotic Resistance Protocols, Methods in Molecular Biology, vol. 1736, https://doi.org/10.100 ...
118 sequencing platforms. Microarray hybridization as an established technology continues to be useful in some settings. For exa ...
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