Antibiotic Resistance Protocols (Methods in Molecular Biology)

160 Mice: BALB/c mice, female and aged 6–8 weeks (Harlan UK Ltd.). Rifampicin, isoniazid and pyrazinamide are obtained from Sig ...

161 Place the culture in an ultrasonic water bath (Branson Ultrasonic B. V.) for 5 min in order to obtain uniformly dis- persed ...

162 Mice are sacrificed at different time points post treatment. Lungs and spleens from mice are removed rapidly by a sterile a ...

163 Then perform the culture in a tenfold serial dilution by adding 0.5 mL of the mixed cell suspension to 4.5 mL of culture fi ...

164 M. tuberculosis tends to grow in clumps even in the detergent- containing 7H9, especially when a culture is incubated with- ...

165 Disease relapse is measured by dividing the numbers of mice with CFU count positive organs with the total numbers of the mi ...

166 ant to first-line drugs. Antimicrob Agents Chemother 60:2476–2483 Hu Y, Liu A, Ortega-Muro F, Alameda-Martin L, Mitchison D ...

167 Stephen H. Gillespie (ed.), Antibiotic Resistance Protocols, Methods in Molecular Biology, vol. 1736, https://doi.org/10.100 ...

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