Antibiotic Resistance Protocols (Methods in Molecular Biology)
34 Colonies should be scored after 24 h of incubation at 37 °C. Determine the average frequency of RifR mutants from three to si ...
35 Classical epifluorescence microscope equipped with a 100× objective, appropriate fluorescent filters, fluorescent lamp, and ...
36 Mount the prepared slide on the epi-fluorescence microscope. Choose the fields with a monolayer of 100–500 cells per field ( ...
37 type strain, overnight in a desired medium, supplemented or not with IPTG. Upon growth to saturation, plate 20 μL of the satu ...
38 Follow the manufacturer’s instructions for the proper sealing of the Gene Frame on the slide. Do not remove the upper plasti ...
39 Drake JW (1991) A constant rate of spontane- ous mutation in DNA-based microbes. Proc Nat Acad Sci USA 88:7160–7164 Lee H, P ...
41 Stephen H. Gillespie (ed.), Antibiotic Resistance Protocols, Methods in Molecular Biology, vol. 1736, https://doi.org/10.1007 ...
42 therapy, and several studies have shown that mycobacteria accumu- late lipid in intracellular bodies and these cells exhibit ...
43 Cryostat to produce 3–5 μm thick frozen tissue sections (see Note 1). Formalin solution. Sucrose. PBS. Quartz coverslip (010 ...
44 Software list: Matlab, Andor Solis. List of optics: LF: Laser line filter (LL01-785, Semrock, USA). EF: Edge filter (LPD02-7 ...
45 Mount the frozen tissue section that is on the quartz coverslip (SPI Supplies, 01015T-AB), directly on top of a quartz slide ...
46 depending on your system, the organism under investigation and the power on the sample plane (see Note 6). Record five spectr ...
47 4 Notes Frozen tissue slicing We have used 5 μm thick formalin-fixed, frozen tissue sec- tions successfully. However, the ti ...
48 duce a final spectrum, a background Raman spectrum is first acquired next to a single bacterium. Once the background is taken ...
49 Additional information on PCA can be found in this article [ 17 ]. We used Matlab to run the PCA and the LOOCV analy- sis (Ma ...
50 sion of modulated Raman spectroscopy. Opt Express 18(11):11382–11395 Baron VO, Chen M, Clark SO et al (2017) Label-free opti ...
51 Stephen H. Gillespie (ed.), Antibiotic Resistance Protocols, Methods in Molecular Biology, vol. 1736, https://doi.org/10.1007 ...
52 and binds to DNA. It will not cross intact membranes, but will eas- ily penetrate compromised membranes that are characterist ...
53 Formaldehyde (Scientific Laboratory Supplies). Cyan ADP flow cytometer (Beckman Coulter) (see Note 2). Sigmaplot graphical a ...
54 appeared within the first order of the logarithmic scale of fluorescence. Analyse the remaining stained samples at the setti ...
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