Methods in Molecular Biology • 16 Enzymes of Molecular Biology

Index 359 unit assay, 239 Vma x, 240 Zn 2÷, 237 BamHI, 134, see also Restriction enzymes BanI, 135, see also Restriction enzymes ...

360 Index Cfr9I, 141, see also Restriction enzymes CfrI, 141, see also Restriction enzymes Chymotrypsin (EC 3.4.21.1 ), 278-281 ...

Index 361 S. typhimurium, 215 storage and stability, 218 substrate, 214 sulfhydryl reagents, 217 T. thermophilus, 215 temperatur ...

362 Index Eco64I, 143, see also Restriction enzymes Eco72I, 143, see also Restriction enzymes Eco81I, 143, see also Restriction ...

Index 363 HgR)I, 146, see also Restriction enzymes HhaI, 146, see also Restriction enzymes HinlI, 146, see also Restriction enzy ...

364 Index Nlal]I, 152, see also Restriction enzymes NlalV, 152, see also Restriction enzymes NotI, 153, see also Restriction enz ...

Index 365 peptide/protein hydrolysate, 326 pH optimum, 324 removal of pyroglutamic acid, 325 specificity, 324 stability, 325 Pfl ...

366 Index stability, 307 Tritirachium album, 305 Proteolytic enzymes, 277-303 chymotrypsin, 278-281 clostripain, 281-283 Edman d ...

Index 367 DNA cellulose, 111 DNA fingerprint, 181 dyes, 124 eicosomer, 111 electrophoresis, 126, 127, 174, 175 enzymoleogy, 109, ...

368 Index dNTPs, 79 inhibitors, 81 Mg 2÷, 79 sodium pyrophosphate, 80 pH and metal ion, 79 polyanions, 80 reducing agent, 81 RNa ...

Index 369 Substrate, see each enzyme Sulfhydryl reagents, see each enzyme T T4 RNA ligase, 223, 225 cations, 224 cofactors, 224 ...

370 Index molecular mass, 291 pH optimum, 291 specificty, 291 stability, 292 Thermus aquaticus, 27 Thermus thermophilus, 27 ThtH ...